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KMID : 0378020030460010052
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New Medical Journal
2003 Volume.46 No. 1 p.52 ~ p.58
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Effect of Hyperthermia on Chemosensitivity of Cisplatin Resistant Human Bladder Cancer Cells
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Abstract
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Cisplatin shows the therapeutic effect on bladder cancer. However the response is often limited due to development of drug resistance. Recently, we established two cisplatin resistance human bladder cancer cell lines, T24R1 and T24R2. In this study we investigated effect of hyperthermia, which was known to increase the chemotherapeutic sensitivity and suppress the tumor cell growth, on chemosensitivity of cisplatin resistant human bladder cancer cell lines in vitro. Cisplation resistant human bladder cancer cell lines T24R1 and T24R2 were used. T24R1 and T24R2 showed 12 and 18 fold resistance to cisplatin compared to parent cell line T24, respectively. Optimal cell number were investigated. Hyperthermia of 43℃ was applied in 2 and 4 hours duration where cisplatin was added in various concentration. The cell growth was measured by MTT colorimetric assay. The effect of hyperthermia on chemosensitivity was compared using dose modification factor (DMF) of IC50 of chemotherapeutic agents. Each experiment was repeated eight times. In 2 and 4hour treatment with hyperthermia, DMFs of cisplatin were 0.9 and 0.6 in T24R1 and 0.8 and 0.5 in T24R2 cells, respectively, which were statistically significant compared to control. These results suggest that the hyperthermia increased the chemosensitivity of cisplatin resistant human bladder cancer cells which could be the clinical tools to overcome the drug resistance. It is necessary to investigate what the mechanism of increasing chemosensitivity
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